The cells were fixed with 4% PFA and 0.2% glutaraldehyde in a buffer containing 30 mM HEPES (pH 7.4), 100 mM NaCl, and 2 mM CaCl 2 for 10 min at room temperature
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When the body loses fluid through these routes without adequate replacement, blood becomes more concentrated
Chowdhury A, Nabila J, AdelusiTemitope I, Wang S
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A 2024 paper in Physiology & Behavior characterised that ipamorelin and the related GHSR-1a agonist anamorelin inhibited cisplatin-induced weight loss in ferrets, framing one route by which the GHSR-1a agonist class engages cachexia research (PMID 39043357)